Native dorsal closure. Time-lapse imaging of a control embryo expressing GFP labeled cadherin. (Mortensen et al., G3. 2018 8(7), 2361-2387, Movie and Fig. 4A).
zip/MyoII is responsible for purse string contractility. Cells that lack GFP-zip/MyoII expression cannot maintain tension and are dramatically stretched by neighboring zip/MyoII-expressing cells. (Franke et al., Curr Bio. 2005 Dec 24; 15(24):2208-2221, Movie S5, Fig. 3A-G).
Segment alignment and filopodial dynamics during dorsal closure. engrailed GAL4 drives the expression of zyxin-GFP and RFP-moe-ABD (F-actin). Movie acquired by U. Serdar Tulu.
Formation of a new, secondary purse string following laser surgical removal of a portion of the leading edge cells and purse string. Embryo expresses sGMCA, a marker of filamentous actin. (Rodriguez-Diaz et al., HFSP Journal. 2008; 2(4):220-237, Supplemental Video 2, Fig. 2).
Closure following laser surgical removal of both canthi. The leading edges flatten during recoil, pause briefly and then resume closure at near native rates. Embryo expresses GFP labeled cadherin. (Wells et al., MBOC. 2014; 25(22):3552-3568, Supplemental Video 1, Fig. 2A).
Volume rendering of the amnioserosa and the encroaching epithelium during dorsal closure in an embryo expressing GFP labeled cadherin to highlight cell-cell junctions. Movie acquired in collaboration with Wes Legant and Eric Betzig. (Chen et al., Science. 2014 Oct 24;346(6208):1257998, Movie S16, Fig. 6C).
Various views of dorsal closure in an embryo expressing sGMCA, a marker of filamentous actin at the plasma membrane. Top left: orthoslice through the center of the amnioserosa. Top right: maximum intensity projection through a thin rectangular slab encompassing the apical surface of the amnioserosa. Bottom right: MIP through a similar slab encompassing the basal surface of the amnioserosa. Bottom left: volume rendering, with the apical surface in blue/green and the basal surface in orange/red. Movie acquired in collaboration with Wes Legant and Eric Betzig. (Chen et al., Science. 2014 Oct 24;346(6208):1257998, Movie S17, Fig. 6E,F).